BMRB Entry 25730

Title:
1H, 15N and 13C backbone assignments of GDP-bound human H-Ras mutant G12V
Deposition date:
2015-07-28
Original release date:
2015-08-27
Authors:
Amin, Nader; Chiarparin, Elisabetta; Coyle, Joe; Nietlispach, Daniel; Williams, Glyn
Citation:

Citation: Amin, Nader; Chiarparin, Elisabetta; Coyle, Joe; Nietlispach, Daniel; Williams, Glyn. "1H, 15N and 13C backbone assignments of GDP-bound human H-Ras mutant G12V"  Biomol. NMR Assign. 10, 121-123.
PubMed: 26373425

Assembly members:

Assembly members:
H-Ras_G12V, polymer, 169 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23b

Data sets:
Data typeCount
13C chemical shifts482
15N chemical shifts160
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1H-Ras g12V1

Entities:

Entity 1, H-Ras g12V 169 residues - Formula weight is not available

1   GLYSERHISMETTHRGLUTYRLYSLEUVAL
2   VALVALGLYALAVALGLYVALGLYLYSSER
3   ALALEUTHRILEGLNLEUILEGLNASNHIS
4   PHEVALASPGLUTYRASPPROTHRILEGLU
5   ASPSERTYRARGLYSGLNVALVALILEASP
6   GLYGLUTHRCYSLEULEUASPILELEUASP
7   THRALAGLYGLNGLUGLUTYRSERALAMET
8   ARGASPGLNTYRMETARGTHRGLYGLUGLY
9   PHELEUCYSVALPHEALAILEASNASNTHR
10   LYSSERPHEGLUASPILEHISGLNTYRARG
11   GLUGLNILELYSARGVALLYSASPSERASP
12   ASPVALPROMETVALLEUVALGLYASNLYS
13   CYSASPLEUALAALAARGTHRVALGLUSER
14   ARGGLNALAGLNASPLEUALAARGSERTYR
15   GLYILEPROTYRILEGLUTHRSERALALYS
16   THRARGGLNGLYVALGLUASPALAPHETYR
17   THRLEUVALARGGLUILEARGGLNHIS

Samples:

sample_1: H-Ras G12V, [U-99% 13C; U-99% 15N], 300 mM; NaCl 100 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks