BMRB Entry 25299

Title:
Backbone fold of Human Small Ubiquitin like Modifier protein-1 (SUMO-1) based on Prot3D-NMR approach.
Deposition date:
2014-10-28
Original release date:
2014-12-01
Authors:
Kumar, Dinesh; jaiswal, Nancy; raikwal, Nisha; Shukla, Vaibhav; Arora, Ashish
Citation:

Citation: Kumar, Dinesh; jaiswal, Nancy; raikwal, Nisha; Shukla, Vaibhav; Arora, Ashish. "Prot3DNMR: A simple and swift NMR strategy for Three-Dimentional structutral determination of proteins."  .

Assembly members:

Assembly members:
entity, polymer, 97 residues, 11149.640 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE80L

Data sets:
Data typeCount
1H chemical shifts440
13C chemical shifts383
15N chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 97 residues - 11149.640 Da.

1   METSERASPGLNGLUALALYSPROSERTHR
2   GLUASPLEUGLYASPLYSLYSGLUGLYGLU
3   TYRILELYSLEULYSVALILEGLYGLNASP
4   SERSERGLUILEHISPHELYSVALLYSMET
5   THRTHRHISLEULYSLYSLEULYSGLUSER
6   TYRCYSGLNARGGLNGLYVALPROMETASN
7   SERLEUARGPHELEUPHEGLUGLYGLNARG
8   ILEALAASPASNHISTHRPROLYSGLULEU
9   GLYMETGLUGLUGLUASPVALILEGLUVAL
10   TYRGLNGLUGLNTHRGLYGLY

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 0.9 ± 0.1 mM; sodium phosphate 200 mM

sample_conditions_1: temperature: 298 K; pH: 6.5; pressure: 1 atm; ionic strength: 200 mM

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA v9.8, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 16611 17536
PDB
DBJ BAB22172 BAB27379 BAB93477 BAC40739 BAE35024
EMBL CAA67898 CAG31129 CAG46944 CAG46953 CAH92616
GB AAB39999 AAB40388 AAB40390 AAC39959 AAC50733
REF NP_001005781 NP_001005782 NP_001009672 NP_001030535 NP_001106146
SP A7WLH8 P63165 P63166 Q2EF74 Q5E9D1
TPG DAA32560
AlphaFold Q2EF74 A7WLH8 P63165 P63166 Q5E9D1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks