BMRB Entry 25167

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for CS domain of human Shq1
Deposition date:
2014-08-20
Original release date:
2015-10-23
Authors:
Singh, Mahavir; Wang, Zhonghua; Cascio, Duilio; Feigon, Juli
Citation:

Citation: Singh, Mahavir; Wang, Zhonghua; Cascio, Duilio; Feigon, Juli. "Structure and interactions of the CS domain of human H/ACA RNP assembly protein Shq1"  J. Mol. Biol. 427, 807-823 (2015).
PubMed: 25553844

Assembly members:

Assembly members:
Human_Shq1_CS_domain, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET46

Data sets:
Data typeCount
13C chemical shifts261
15N chemical shifts86
1H chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Shq1 CS domain1

Entities:

Entity 1, Human Shq1 CS domain 133 residues - Formula weight is not available

GSTAIGMKETAAAKFERQHMDSPDLGTGGGSGDDDDK (residues -37 to -1) are from the vector after GST tag cleavage

1   GLYSERTHRALAILEGLYMETLYSGLUTHR
2   ALAALAALALYSPHEGLUARGGLNHISMET
3   ASPSERPROASPLEUGLYTHRGLYGLYGLY
4   SERGLYASPASPASPASPLYSMETLEUTHR
5   PROALAPHEASPLEUSERGLNASPPROASP
6   PHELEUTHRILEALAILEARGVALPROTYR
7   ALAARGVALSERGLUPHEASPVALTYRPHE
8   GLUGLYSERASPPHELYSPHETYRALALYS
9   PROTYRPHELEUARGLEUTHRLEUPROGLY
10   ARGILEVALGLUASNGLYSERGLUGLNGLY
11   SERTYRASPALAASPLYSGLYILEPHETHR
12   ILEARGLEUPROLYSGLUTHRPROGLYGLN
13   HISPHEGLUGLYLEUASNMETLEUTHRALA
14   LEULEUALA

Samples:

sample_1: Human Shq1 CS domain, [U-100% 15N], 0.2 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; H2O 93%; D2O 7%; sodium azide 0.02%

sample_2: Human Shq1 CS domain, [U-100% 13C; U-100% 15N], 0.2 – 1.0 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 2 mM; H2O 93%; D2O 7%; sodium azide 0.02%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1

Software:

TOPSPIN vBruker, Bruker Biospin - collection, data analysis, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 19910
PDB
GB AAH17204 AAH17274 AAH25270 AAH32671 AAH39830
REF NP_060600 XP_001082636 XP_001141951 XP_001493794 XP_003132372
SP Q6PI26
AlphaFold Q6PI26

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks