BMRB Entry 19733
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19733
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Citation: Callon, Morgane; Burmann, Bjorn; Hiller, Sebastian. "Structural Mapping of a Chaperone-Substrate Interaction Surface." Angew. Chem. Int. Ed. Engl. ., .-. (2014).
PubMed: 24700611
Assembly members:
Escherichia_Coli_Skp, polymer, 162 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b
Entity Sequences (FASTA):
Escherichia_Coli_Skp: MGSSHHHHHHSSGLVPRGSH
MADKIAIVNMGSLFQQVAQK
TGVSNTLENEFKGRASELQR
METDLQAKMKKLQSMKAGSD
RTKLEKDVMAQRQTFAQKAQ
AFEQDRARRSNEERGKLVTR
IQTAVKSVANSQDIDLVVDA
NAVAYNSSDVKDITADVLKQ
VK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 14 |
| 1H chemical shifts | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Skp, chain 1 | 1 |
| 2 | Skp, chain 2 | 1 |
| 3 | Skp, chain 3 | 1 |
Entities:
Entity 1, Skp, chain 1 162 residues - Formula weight is not available
native processed E. coli Skp (residues 21 - 161) hexahistidin-tag and thrombin cleavage site
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | ASP | LYS | ILE | ALA | ILE | VAL | ASN | MET | ||||
| 4 | GLY | SER | LEU | PHE | GLN | GLN | VAL | ALA | GLN | LYS | ||||
| 5 | THR | GLY | VAL | SER | ASN | THR | LEU | GLU | ASN | GLU | ||||
| 6 | PHE | LYS | GLY | ARG | ALA | SER | GLU | LEU | GLN | ARG | ||||
| 7 | MET | GLU | THR | ASP | LEU | GLN | ALA | LYS | MET | LYS | ||||
| 8 | LYS | LEU | GLN | SER | MET | LYS | ALA | GLY | SER | ASP | ||||
| 9 | ARG | THR | LYS | LEU | GLU | LYS | ASP | VAL | MET | ALA | ||||
| 10 | GLN | ARG | GLN | THR | PHE | ALA | GLN | LYS | ALA | GLN | ||||
| 11 | ALA | PHE | GLU | GLN | ASP | ARG | ALA | ARG | ARG | SER | ||||
| 12 | ASN | GLU | GLU | ARG | GLY | LYS | LEU | VAL | THR | ARG | ||||
| 13 | ILE | GLN | THR | ALA | VAL | LYS | SER | VAL | ALA | ASN | ||||
| 14 | SER | GLN | ASP | ILE | ASP | LEU | VAL | VAL | ASP | ALA | ||||
| 15 | ASN | ALA | VAL | ALA | TYR | ASN | SER | SER | ASP | VAL | ||||
| 16 | LYS | ASP | ILE | THR | ALA | ASP | VAL | LEU | LYS | GLN | ||||
| 17 | VAL | LYS |
Samples:
sample_1: Escherichia_Coli_Skp (trimer), [U-13CE, U-1H]-Met; [U-13CB; U-1HB]-Ala; [U-2H; U-15N], 0.7 mM; H2O 90%; D2O 10%; MES 25 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Keller and Wuthrich - chemical shift assignment, data analysis
TOPSPIN, Bruker Biospin - collection
PROSA, Guntert - processing
NMR spectrometers:
- Bruker AscendII 700 MHz
Related Database Links:
| PDB | |
| BMRB | 19730 19410 19409 19407 19407 19408 19409 19730 |
| UNP | P0AEU7 |
| DBJ | BAA77853 BAB33603 BAG75701 BAI23539 BAI29054 |
| EMBL | CAA38567 CAP74747 CAQ30692 CAQ97065 CAR01553 |
| GB | AAA24630 AAB08607 AAC73289 AAG54480 AAN41830 |
| REF | NP_308207 NP_414720 NP_706123 WP_000758954 WP_000758955 |
| SP | P0AEU7 P0AEU8 P0AEU9 P0AEV0 Q325W2 |
| AlphaFold | P11457 P0AEV0 P0AEU7 P0AEU8 P0AEU9 Q325W2 |