BMRB Entry 19638

Title:
Solution structure of cytochrome c Y67H
Deposition date:
2013-11-26
Original release date:
2014-10-27
Authors:
Lan, W.; Wang, Z.; Ying, T.; Yang, Z.; Zhang, X.; Wu, H.; Liu, M.; Tian, X.
Citation:

Citation: Lan, Wenxian; Wang, Zhonghua; Yang, Zhongzheng; Ying, Tianlei; Zhang, Xu; Tan, Xiangshi; Liu, Maili; Cao, Chunyang; Huang, Zhong-Xian. "Structural Basis for Cytochrome c Y67H Mutant to Function as a Peroxidase"  Plos One 9, e107305-e107305 (2014).
PubMed: 25210769

Assembly members:

Assembly members:
entity_1, polymer, 108 residues, 11988.768 Da.
entity_HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBTR2

Data sets:
Data typeCount
1H chemical shifts667

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c Y67H1
2HEME C2

Entities:

Entity 1, cytochrome c Y67H 108 residues - 11988.768 Da.

1   THRGLUPHELYSALAGLYSERALALYSLYS
2   GLYALATHRLEUPHELYSTHRARGCYSLEU
3   GLNCYSHISTHRVALGLULYSGLYGLYPRO
4   HISLYSVALGLYPROASNLEUHISGLYILE
5   PHEGLYARGHISSERGLYGLNALAGLUGLY
6   TYRSERTYRTHRASPALAASNILELYSLYS
7   ASNVALLEUTRPASPGLUASNASNMETSER
8   GLUHISLEUTHRASNPROALALYSTYRILE
9   PROGLYTHRLYSMETALAPHEGLYGLYLEU
10   LYSLYSGLULYSASPARGASNASPLEUILE
11   THRTYRLEULYSLYSALATHRGLU

Entity 2, HEME C - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytc c y67h 1 mM; phosphate buffer NA 50 mM

sample_2: cytc c y67h 1 mM; phosphate buffer NA 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1isotropicsample_conditions_1
TOCSYsample_2isotropicsample_conditions_1
DQCOSYsample_2isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Agilent NMR 600 MHz

Related Database Links:

BMRB 131 15301 17845 17846 17903 17904
PDB
DBJ GAA24396
EMBL CAA24605 CAA89576 CAY80764
GB AAA62856 AAA88751 AAB59344 AHY79034 AJP39735
PRF 1409323A
REF NP_012582
SP P00044
TPG DAA08835
AlphaFold P00044