BMRB Entry 17909

Title:
Resonance assignments of human Steroidogenic acute regulatory-related lipid transfer domain protein 5
Deposition date:
2011-09-01
Original release date:
2011-10-03
Authors:
Lorin, Aurelien; Letourneau, Danny; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre
Citation:

Citation: Lorin, Aurelien; Letourneau, Danny; Lefebvre, Andree; LeHoux, Jean-Guy; Lavigne, Pierre. "(1)H, (13)C, and (15)N backbone chemical shift assignments of StAR-related lipid transfer domain protein 5 (STARD5)."  Biomol. NMR Assignments 7, 21-24 (2013).
PubMed: 22392336

Assembly members:

Assembly members:
StarD5, polymer, 213 residues, 23797.1169 Da.

Natural source:

Natural source:   Common Name: E. coli BL21(DE3)   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET3A

Data sets:
Data typeCount
13C chemical shifts705
15N chemical shifts190
1H chemical shifts344

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1StarD51

Entities:

Entity 1, StarD5 213 residues - 23797.1169 Da.

1   METASPPROALALEUALAALAGLNMETSER
2   GLUALAVALALAGLULYSMETLEUGLNTYR
3   ARGARGASPTHRALAGLYTRPLYSILECYS
4   ARGGLUGLYASNGLYVALSERVALSERTRP
5   ARGPROSERVALGLUPHEPROGLYASNLEU
6   TYRARGGLYGLUGLYILEVALTYRGLYTHR
7   LEUGLUGLUVALTRPASPCYSVALLYSPRO
8   ALAVALGLYGLYLEUARGVALLYSTRPASP
9   GLUASNVALTHRGLYPHEGLUILEILEGLN
10   SERILETHRASPTHRLEUCYSVALSERARG
11   THRSERTHRPROSERALAALAMETLYSLEU
12   ILESERPROARGASPPHEVALASPLEUVAL
13   LEUVALLYSARGTYRGLUASPGLYTHRILE
14   SERSERASNALATHRHISVALGLUHISPRO
15   LEUCYSPROPROLYSPROGLYPHEVALARG
16   GLYPHEASNHISPROCYSGLYCYSPHECYS
17   GLUPROLEUPROGLYGLUPROTHRLYSTHR
18   ASNLEUVALTHRPHEPHEHISTHRASPLEU
19   SERGLYTYRLEUPROGLNASNVALVALASP
20   SERPHEPHEPROARGSERMETTHRARGPHE
21   TYRALAASNLEUGLNLYSALAVALLYSGLN
22   PHEHISGLU

Samples:

StarD5_1: StarD5, [U-13C; U-15N; U-2H], 1.0 mM; H2O 90%; D2O 10%

CondSet1: ionic strength: 0.050 M; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
3D HNCACBStarD5_1isotropicCondSet1
Expt_4 (H[N[co[{CA|ca[C]}]]])StarD5_1isotropicCondSet1
3D HNCACBStarD5_1isotropicCondSet1
3D HNCOStarD5_1isotropicCondSet1
Expt_49 (H{[N]+[HA]})StarD5_1isotropicCondSet1
Expt_53 (H{[N]+[HA]})StarD5_1isotropicCondSet1

Software:

ANALYSIS v2.1, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian UnityInova 600 MHz

Related Database Links:

UniProt Q9NSY2
BMRB 18721
PDB
EMBL CAH91861
GB AAH04365 AAL89654 AIC52409 EAW99097 EHH27540
REF NP_001126078 NP_001247845 NP_871629 XP_003281060 XP_003810787
SP Q5R8P9 Q9NSY2
AlphaFold P59094 Q5R8P9 Q9NSY2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks