BMRB Entry 17846

Title:
Backbone 1H, 13C, and 15N chemical shift assignments of reduced yeast iso-1 cytochrome c
Deposition date:
2011-08-09
Original release date:
2012-03-14
Authors:
Volkov, Alexander; Van de Water, Karen; Vanwetswinkel, Sophie; van Nuland, Nico
Citation:

Citation: Volkov, Alexander; Vanwetswinkel, Sophie; Van de Water, Karen; van Nuland, Nico. "Redox-dependent conformational changes in eukaryotic cytochromes revealed by paramagnetic NMR spectroscopy"  J. Biomol. NMR 52, 245-256 (2012).
PubMed: 22318343

Assembly members:

Assembly members:
yCc, polymer, 108 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pUCcc

Data sets:
Data typeCount
13C chemical shifts312
15N chemical shifts221
1H chemical shifts238

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1yCc1
2HEC2

Entities:

Entity 1, yCc 108 residues - Formula weight is not available

1   ALAGLUPHELYSALAGLYSERALALYSLYS
2   GLYALATHRLEUPHELYSTHRARGCYSLEU
3   GLNCYSHISTHRVALGLULYSGLYGLYPRO
4   HISLYSVALGLYPROASNLEUHISGLYILE
5   PHEGLYARGHISSERGLYGLNALAGLUGLY
6   TYRSERTYRTHRASPALAASNILELYSLYS
7   ASNVALLEUTRPASPGLUASNASNMETSER
8   GLUTYRLEUTHRASNPROLYSLYSTYRILE
9   PROGLYTHRLYSMETALAPHEGLYGLYLEU
10   LYSLYSGLULYSASPARGASNASPLEUILE
11   THRTYRLEULYSLYSALATHRGLU

Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: yCc, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 95%; D2O 5%

sample_2: yCc, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 20 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 115 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 15 mM; pH: 6.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_2
3D NOESY-HSQCsample_2isotropicsample_conditions_2

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian Uniform NMR System 800 MHz
  • Varian Uniform NMR System 600 MHz

Related Database Links:

BMRB 131 15301 1719 1720 17845 17903 17904 190 19638 345 346
PDB
DBJ GAA24396
EMBL CAA24605 CAA89576 CAY80764
GB AAA62856 AAA88751 AAB59344 AHY79034 AJP39735
PRF 1409323A
REF NP_012582
SP P00044
TPG DAA08835
AlphaFold P00044

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks