BMRB Entry 17664

Title:
HVS ORF57 103-120 backbone assignment
Deposition date:
2011-05-25
Original release date:
2011-09-01
Authors:
Tunnicliffe, Richard; Hautbergue, Guillaume; Wilson, Stuart; Golovanov, Alexander
Citation:

Citation: Tunnicliffe, Richard; Hautbergue, Guillaume; Kalra, Priti; Jackson, Brian; Whitehouse, Adrian; Wilson, Stuart; Golovanov, Alexander. "Structural basis for the recognition of cellular mRNA export factor REF by herpes viral proteins HSV-1 ICP27 and HVS ORF57."  PLoS Pathog. 7, .-. (2011).
PubMed: 21253573

Assembly members:

Assembly members:
HVS_ORF57, polymer, 23 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Herpesvirus saimiri   Taxonomy ID: 10381   Superkingdom: Viruses   Kingdom: not available   Genus/species: Herpesvirus saimiri

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HVS_ORF57: GPLGSSCKTSWADRVREAAA QRR

Data sets:
Data typeCount
13C chemical shifts85
15N chemical shifts23
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ORF57 103-1201

Entities:

Entity 1, ORF57 103-120 23 residues - Formula weight is not available

N-terminal sequence GPLGS is non-native from cloning

1   GLYPROLEUGLYSERSERCYSLYSTHRSER
2   TRPALAASPARGVALARGGLUALAALAALA
3   GLNARGARG

Samples:

sample_1: HVS ORF57, [U-95% 13C; U-95% 15N], 1.0 ± 0.05 mM; H20 90%; D20 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

SPARKY v3.113, Goddard - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 16698 17663
PDB
EMBL CAA45680 CAC84353 CAC84354
GB AAA46125 AAA66558
PIR WMBEHA
REF NP_040259
SP P13199
AlphaFold P13199

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks