BMRB Entry 17460

Title:
The Stability and Dynamics of Ribosomal Protein L9: Investigations of a Molecular Strut by Amide Proton Exchange and Circular Dichroism
Deposition date:
2011-02-11
Original release date:
2016-06-09
Authors:
Lillemoen, Jarle; Cameron, Christopher; Hoffman, David
Citation:

Citation: Lillemoen, Jarle; Cameron, Christopher; Hoffman, David. "The Stability and Dynamics of Ribosomal Protein L9: Investigations of a Molecular Strut by Amide Proton Exchange and Circular Dichroism"  J. Mol. Biol. 268, 482-493 (1997).

Assembly members:

Assembly members:
L9, polymer, 149 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: B. stearothermophilus   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: ns

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L91

Entities:

Entity 1, L9 149 residues - Formula weight is not available

1   METLYSVALILEPHELEULYSASPVALLYS
2   GLYLYSGLYLYSLYSGLYGLUILELYSASN
3   VALALAASPGLYTYRALAASNASNPHELEU
4   PHELYSGLNGLYLEUALAILEGLUALATHR
5   PROALAASNLEULYSALALEUGLUALAGLN
6   LYSGLNLYSGLUGLNARGGLNALAALAGLU
7   GLULEUALAASNALALYSLYSLEULYSGLU
8   GLNLEUGLULYSLEUTHRVALTHRILEPRO
9   ALALYSALAGLYGLUGLYGLYARGLEUPHE
10   GLYSERILETHRSERLYSGLNILEALAGLU
11   SERLEUGLNALAGLNHISGLYLEULYSLEU
12   ASPLYSARGLYSILEGLULEUALAASPALA
13   ILEARGALALEUGLYTYRTHRASNVALPRO
14   VALLYSLEUHISPROGLUVALTHRALATHR
15   LEULYSVALHISVALTHRGLUGLNLYS

Samples:

sample_1: L9, [U-100% 15N], ns mM; D2O 100%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
1H-2H HSMQC exchangesample_1isotropicsample_conditions_1

Software:

MOLSCRIPT, Kraulis, 1991 - ribbon drawing

MIDAS, Ferrin et al., 1988; Huang et al., 1991 - figure representation

NMR spectrometers:

  • Varian INOVA 500 MHz