BMRB Entry 17220

Title:
Energetics by NMR: Site-specific binding in a positively cooperative system
Deposition date:
2010-09-29
Original release date:
2010-11-10
Authors:
Tochtrop, Gregory; Richter, Klaus; Tang, Changguo; Toner, James; Covey, Douglas; Cistola, David
Citation:

Citation: Tochtrop, Gregory; Richter, Klaus; Tang, Changguo; Toner, James; Covey, Douglas; Cistola, David. "Energetics by NMR: Site-specific binding in a positively cooperative system"  Proc. Natl. Acad. Sci. U.S.A. 99, 1847-1852 (2002).
PubMed: 11854486

Assembly members:

Assembly members:
BABP, polymer, 127 residues, 465.623 Da.
GLYCOCHOLIC ACID, non-polymer, 465.623 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMON-hIBABP

Data sets:
  • chemical rates
Data typeCount
binding constants3
kinetic rates2

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BABP1
2GCH2

Entities:

Entity 1, BABP 127 residues - 465.623 Da.

1   ALAPHETHRGLYLYSPHEGLUMETGLUSER
2   GLULYSASNTYRASPGLUPHEMETLYSLEU
3   LEUGLYILESERSERASPVALILEGLULYS
4   ALAARGASNPHELYSILEVALTHRGLUVAL
5   GLNGLNASPGLYGLNASPPHETHRTRPSER
6   GLNHISTYRSERGLYGLYHISTHRMETTHR
7   ASNLYSPHETHRVALGLYLYSGLUSERASN
8   ILEGLNTHRMETGLYGLYLYSTHRPHELYS
9   ALATHRVALGLNMETGLUGLYGLYLYSLEU
10   VALVALASNPHEPROASNTYRHISGLNTHR
11   SERGLUILEVALGLYASPLYSLEUVALGLU
12   VALSERTHRILEGLYGLYVALTHRTYRGLU
13   ARGVALSERLYSARGLEUALA

Entity 2, GCH - C26 H43 N O6 - 465.623 Da.

1   GCH

Samples:

sample_1: GCH, [U-13C; U-15N], 0 – 10 mM; BABP 1.85 mM; potassium phosphate 20 mM; potassium chloride 135 mM; sodium chloride 10 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.20; pressure: ambient atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D HCACOsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - data analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
BMRB 19843
DBJ BAI46829
EMBL CAA62415
GB AAB82751 AAH22489 ABA12611 ADQ32819 AIC54371
REF NP_001035532 NP_001124430 NP_001436 XP_001083748 XP_001083965
SP P51161
AlphaFold P51161