BMRB Entry 17184

Title:
Lactose Binding to Galectin-1 Modulates Structural Dynamics, Increases Conformational Entropy, and Occurs with Apparent Negative Cooperativity
Deposition date:
2010-09-10
Original release date:
2010-11-10
Authors:
Nesmelova, Irina; Ermakova, Elena; Daragan, Vladimir; Pang, Mabel; Menendez, Margarita; Lagartera, Laura; Solis, Dolores; Baum, Linda; Mayo, Kevin
Citation:

Citation: Nesmelova, Irina; Ermakova, Elena; Daragan, Vladimir; Pang, Mabel; Menendez, Margarita; Lagartera, Laura; Solis, Dolores; Baum, Linda; Mayo, Kevin. "Lactose Binding to Galectin-1 Modulates Structural Dynamics, Increases Conformational Entropy, and Occurs with Apparent Negative Cooperativity"  J. Mol. Biol. 397, 1209-1230 (2010).
PubMed: 20184898

Assembly members:

Assembly members:
gal1, polymer, 135 residues, Formula weight is not available
LAT, SACCHARIDE, 342.296 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Data sets:
  • binding constants
  • pKa values
Data typeCount
binding constants2
pKa values1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gal11
2LAT2

Entities:

Entity 1, gal1 135 residues - Formula weight is not available

1   METALACYSGLYLEUVALALASERASNLEU
2   ASNLEULYSPROGLYGLUCYSLEUARGVAL
3   ARGGLYGLUVALALAPROASPALALYSSER
4   PHEVALLEUASNLEUGLYLYSASPSERASN
5   ASNLEUCYSLEUHISPHEASNPROARGPHE
6   ASNALAHISGLYASPALAASNTHRILEVAL
7   CYSASNSERLYSASPGLYGLYALATRPGLY
8   THRGLUGLNARGGLUALAVALPHEPROPHE
9   GLNPROGLYSERVALALAGLUVALCYSILE
10   THRPHEASPGLNALAASNLEUTHRVALLYS
11   LEUPROASPGLYTYRGLUPHELYSPHEPRO
12   ASNARGLEUASNLEUGLUALAILEASNTYR
13   METALAALAASPGLYASPPHELYSILELYS
14   CYSVALALAPHEASP

Entity 2, LAT - 342.296 Da.

1   LAT

Samples:

sample_1: gal1, [U-13C; U-15N], 34 – 2000 uM; potassium phosphate buffer 20 mM; deuterated DTT 2 mM; LAT0 – 100 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: ambient atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - visualization

NMR spectrometers:

  • Varian Unity Inova 800 MHz

Related Database Links:

BMRB 15800
PDB
DBJ BAC77389 BAG35095 BAG72608
EMBL CAA32938 CAA33328 CAG30397 CAH92239 CAK54541
GB AAA36170 AAB00777 AAH01693 AAH20675 AAP35421
PRF 1409286A 1713410A
REF NP_001126310 NP_001135409 NP_001162098 NP_002296 XP_001162104
SP P09382 Q5R7M1
AlphaFold P09382 Q5R7M1