BMRB Entry 17102

Name: NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domain
Published: 2010-11-10

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17102

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domain

Deposition date:

2010-08-02

Original release date:

2010-11-10

Authors:

Millet, Oscar; Bernado, Pau; Garcia, Jesus; Rizo, Josep; Pons, Miquel

Citation:

Citation: Millet, Oscar; Bernado, Pau; Garcia, Jesus; Rizo, Josep; Pons, Miquel. "NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domain" FEBS Lett. 516, 93-96 (2002).
PubMed: 11959110

Assembly members:

Assembly members:
C2A, polymer, 143 residues, 40.078 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2A: GSPGIGGGGGGILDSMVEKL GKLQYSLDYDFQNNQLLVGI IQAAELPALDMGGTSDPYVK VFLLPDKKKKFETKVHRKTL NPVFNEQFTFKVPYSELGGK TLVMAVYDFNRFSKHDIIGE FKVPMNTVDFGHVTEEWRDL QSA

Data sets:

chemical_rates
- Chemical_rate_list

Data type	Count
kinetic rates	3

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2A	1
2	CA	2

Entities:

Entity 1, C2A 143 residues - 40.078 Da.

1

GLY

SER

PRO

GLY

ILE

GLY

2

GLY

ILE

LEU

ASP

SER

MET

VAL

GLU

LYS

LEU

3

GLY

LYS

LEU

GLN

TYR

SER

LEU

ASP

TYR

ASP

4

PHE

GLN

ASN

GLN

LEU

VAL

GLY

ILE

5

ILE

GLN

ALA

GLU

LEU

PRO

ALA

LEU

ASP

6

MET

GLY

THR

SER

ASP

PRO

TYR

VAL

LYS

7

VAL

PHE

LEU

PRO

ASP

LYS

8

PHE

GLU

THR

LYS

VAL

HIS

ARG

LYS

THR

LEU

9

ASN

PRO

VAL

PHE

ASN

GLU

GLN

PHE

THR

PHE

10

LYS

VAL

PRO

TYR

SER

GLU

LEU

GLY

LYS

11

THR

LEU

VAL

MET

ALA

VAL

TYR

ASP

PHE

ASN

12

ARG

PHE

SER

LYS

HIS

ASP

ILE

GLY

GLU

13

PHE

LYS

VAL

PRO

MET

ASN

THR

VAL

ASP

PHE

14

GLY

HIS

VAL

THR

GLU

TRP

ARG

ASP

LEU

15

GLN

SER

ALA

Entity 2, CA - Ca - 40.078 Da.

1

CA

Samples:

sample_1: C2A, [U-15N], 0.93 mM; CA 0.198 mM; EDTA 0.198 mM; Tris 20 mM; sodium chloride 100 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-15N NOESY-HSQC	sample_1	isotropic	sample_conditions_1
RC-CPMG	sample_1	isotropic	sample_conditions_1

Software:

CurveFit, GraphPad Software, Inc. - relaxation rates and their errors

Microcal_Origin, (Microcal Software) - data analysis

Mathematica, (Wolfram Research) - data analysis

NMR spectrometers:

Varian INOVA 600 MHz
Bruker Avance 800 MHz

BMRB	15785 15952 4039 4041 4166 4167
PDB	1BYN 2K45 2K4A 2K8M 2KI6 3F00 3F01 3F04 3F05
GB	AAK15077 ELW70522 EPY85163 KFO26677 KFP32561
REF	XP_005976391 XP_010164835 XP_010202276