BMRB Entry 16670

Title:
NMR structure of the apoptosis- and inflammation-related NALP1 pyrin domain
Deposition date:
2010-01-08
Original release date:
2010-01-19
Authors:
Hiller, Sebastian; Kohl, Andreas; Fiorito, Francesco; Herrmann, Torsten; Wider, Gerhard; Tschopp, Jurg; Grutter, Markus; Wuthrich, Kurt
Citation:

Citation: Hiller, Sebastian; Kohl, Andreas; Fiorito, Francesco; Herrmann, Torsten; Wider, Gerhard; Tschopp, Jurg; Grutter, Markus; Wuthrich, Kurt. "NMR structure of the apoptosis- and inflammation-related NALP1 pyrin domain"  Structure 11, 1190-1205 (2003).
PubMed: 14527388

Assembly members:

Assembly members:
NALP1_PYD, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: peT20b

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts102
1H chemical shifts600

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NALP1_PYD1

Entities:

Entity 1, NALP1_PYD 97 residues - Formula weight is not available

1   METALAGLYGLYALATRPGLYARGLEUALA
2   CYSTYRLEUGLUPHELEULYSLYSGLUGLU
3   LEULYSGLUPHEGLNLEULEULEUALAASN
4   LYSALAHISSERARGSERSERSERGLYGLU
5   THRPROALAGLNPROGLULYSTHRSERGLY
6   METGLUVALALASERTYRLEUVALALAGLN
7   TYRGLYGLUGLNARGALATRPASPLEUALA
8   LEUHISTHRTRPGLUGLNMETGLYLEUARG
9   SERLEUCYSALAGLNALAGLNGLUGLYALA
10   GLYHISSERLEUGLUHISHIS

Samples:

sample_1: NALP1_PYD, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT, [U-100% 2H], 20 mM; CHAPS 1 mM; sodium azide 0.02%; EDTA 0.1 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

PDB
SWS Q9C000
UNP Q9C000
DBJ BAA76770 BAG11260
GB AAG15254 AAG30288 AAH51787 AAK00748 AAK00749
REF NP_001028225 NP_055737 NP_127497 NP_127499 NP_127500
SP Q9C000
AlphaFold Q9Y2E0 Q9C000

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks