BMRB Entry 16494

Title:
Backbone and side chain 1H chemical shift assignments for acidic fibrobast growth factor
Deposition date:
2009-09-15
Original release date:
2009-10-06
Authors:
Pineda-Lucena, Antonio; Jimenez, M. Angeles; Lozano, Rosa; Nieto, Jose; Santoro, Jorge; Rico, Manuel; Gimenez-Gallego, Guillermo
Citation:

Citation: Pineda-Lucena, Antonio; Jimenez, M. Angeles; Nieto, Jose; Santoro, Jorge; Rico, Manuel; Gimenez-Gallego, Guillermo. "1H-NMR assignment and solution structure of human acidic fibroblast growth factor activated by inositol hexasulfate"  J. Mol. Biol. 242, 81-98 (1994).
PubMed: 7521397

Assembly members:

Assembly members:
aFGF, polymer, 132 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMG47

Data sets:
Data typeCount
1H chemical shifts847

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1acidic fibroblast growth factor1

Entities:

Entity 1, acidic fibroblast growth factor 132 residues - Formula weight is not available

Protein construct lacks the 22 N-terminal residues.

1   LYSLYSPROLYSLEULEUTYRCYSSERASN
2   GLYGLYHISPHELEUARGILELEUPROASP
3   GLYTHRVALASPGLYTHRARGASPARGSER
4   ASPGLNHISILEGLNLEUGLNLEUSERALA
5   GLUSERVALGLYGLUVALTYRILELYSSER
6   THRGLUTHRGLYGLNTYRLEUALAMETASP
7   THRASPGLYLEULEUTYRGLYSERGLNTHR
8   PROASNGLUGLUCYSLEUPHELEUGLUARG
9   LEUGLUGLUASNHISTYRASNTHRTYRILE
10   SERLYSLYSHISALAGLULYSASNTRPPHE
11   VALGLYLEULYSLYSASNGLYSERCYSLYS
12   ARGGLYPROARGTHRHISTYRGLYGLNLYS
13   ALAILELEUPHELEUPROLEUPROVALSER
14   SERASP

Samples:

sample_1: aFGF1 – 2 mM; sodium phosphate 10 mM; TSP0.1 – 0.2 mM; H2O 90%; D2O 10%

sample_2: aFGF1 – 2 mM; sodium phosphate 10 mM; TSP0.1 – 0.2 mM; D2O 100%

sample_conditions_1: ionic strength: 10 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

UXNMR, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AMX 600 MHz