BMRB Entry 16048

Title:
human brain-type fatty acid binding protein (hb-FABP bound to oleic acid (OLA))
Deposition date:
2008-12-01
Original release date:
2009-03-11
Authors:
Oeemig, Jesper; Jorgensen, Mathilde; Hansen, Mikka; Petersen, Evamaria; Duroux, Laurent; Wimmer, Reinhard
Citation:

Citation: Oeemig, Jesper; Jorgensen, Mathilde; Hansen, Mikka; Petersen, Evamaria; Duroux, Laurent; Wimmer, Reinhard. "Backbone and sidechain 1H, 13C and 15N resonance assignments of the human brain-type fatty acid binding protein (FABP7) in its apo form and the holo forms binding to DHA, oleic acid, linoleic acid and elaidic acid"  Biomol. NMR Assignments 3, 89-93 (2009).
PubMed: 19636954

Assembly members:

Assembly members:
hb-FABP, polymer, 131 residues, 282.461 Da.
OLA, non-polymer, 282.461 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts388
15N chemical shifts141
1H chemical shifts863

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hb-FABP1
2Oleic acid2

Entities:

Entity 1, hb-FABP 131 residues - 282.461 Da.

1   VALGLUALAPHECYSALATHRTRPLYSLEU
2   THRASNSERGLNASNPHEASPGLUTYRMET
3   LYSALALEUGLYVALGLYPHEALATHRARG
4   GLNVALGLYASNVALTHRLYSPROTHRVAL
5   ILEILESERGLNGLUGLYASPLYSVALVAL
6   ILEARGTHRLEUSERTHRPHELYSASNTHR
7   GLUILESERPHEGLNLEUGLYGLUGLUPHE
8   ASPGLUTHRTHRALAASPASPARGASNCYS
9   LYSSERVALVALSERLEUASPGLYASPLYS
10   LEUVALHISILEGLNLYSTRPASPGLYLYS
11   GLUTHRASNPHEVALARGGLUILELYSASP
12   GLYLYSMETVALMETTHRLEUTHRPHEGLY
13   ASPVALVALALAVALARGHISTYRGLULYS
14   ALA

Entity 2, Oleic acid - C18 H34 O2 - 282.461 Da.

1   OLA

Samples:

sample_1: hb-FABP, [U-98% 13C; U-98% 15N], 0.85 mM; D2O 5%; H2O 95%; DSS 1 mM; TRIS 10 mM; Oleic acid 8.3 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 7.4; pressure: 1 atm; temperature: 298.1 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CARA v1.5.5, CARA - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 16042 16046 16047 16049
PDB
DBJ BAA23324 BAA23645 BAD92052 BAE90445 BAF82525
EMBL CAA05773 CAC21646 CAG33338
GB AAB87141 AAD00507 AAH12299 AAY17257 ABM82363
REF NP_001020400 NP_001244643 NP_001270567 NP_001437 XP_002746967
SP O15540
AlphaFold O15540

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks