BMRB Entry 15818

Title:
Type III antifreeze protein isoform HPLC-12
Deposition date:
2008-06-23
Original release date:
2009-02-03
Authors:
Siemer, Ansgar; McDermott, Ann
Citation:

Citation: Siemer, Ansgar; McDermott, Ann. "Solid-State NMR on a Type III Antifreeze Protein in the Presence of Ice"  J. Am. Chem. Soc. 130, 17394-17399 (2008).
PubMed: 19053456

Assembly members:

Assembly members:
type_III_AFP_isoform_HPLC-12, polymer, 74 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: ocean pout   Taxonomy ID: 8199   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Macrozoarces americanus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-20b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
type_III_AFP_isoform_HPLC-12: MNQASVVANQLIPINTALTL VMMRSEVVTPVGIPAEDIPR LVSMQVNRAVPLGTTLMPDM VKGYAALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 74 residues - Formula weight is not available

1   METASNGLNALASERVALVALALAASNGLN
2   LEUILEPROILEASNTHRALALEUTHRLEU
3   VALMETMETARGSERGLUVALVALTHRPRO
4   VALGLYILEPROALAGLUASPILEPROARG
5   LEUVALSERMETGLNVALASNARGALAVAL
6   PROLEUGLYTHRTHRLEUMETPROASPMET
7   VALLYSGLYTYRALAALALEUGLUHISHIS
8   HISHISHISHIS

Samples:

sample_1: H2O 100%; type III AFP isoform HPLC-12, [U-100% 13C; U-100% 15N], 1.4 mg

sample_conditions_1: pressure: 1 atm; temperature: 258 K

Experiments:

NameSampleSample stateSample conditions
2D 13C - 13C DARRsample_1isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - collection, processing

CARA v1.8.4, Keller and Wuthrich - data analysis

NMR spectrometers:

  • Bruker Avance 750 MHz

Related Database Links:

UniProt P19614
BMRB 15817
PDB
GB ALL26675
SP P19614
AlphaFold P19614 P19614