BMRB Entry 15767

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15767

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Title:
Backbone Resonance Assignments of the N-terminal half of hepatitis core protein
Deposition date:
2008-05-12
Original release date:
2008-11-03
Authors:
DUVIGNAUD, Jean-Baptiste; SAVARD, Christian; FROMENTIN, Remi; MAJEAU, Nathalie; LECLERC, Denis; GAGNE, Stephane
Citation:

Citation: DUVIGNAUD, Jean-Baptiste; SAVARD, Christian; FROMENTIN, Remi; MAJEAU, Nathalie; LECLERC, Denis; GAGNE, Stephane. "Structure and dynamics of the N-terminal half of hepatitis C virus core protein: an intrinsically unstructured protein"  Biochem. Biophys. Res. Commun. 378, 27-31 (2009).
PubMed: 18992225

Assembly members:

Assembly members:
HCV_core_protein, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: Viruses   Kingdom: not available   Genus/species: Hepacivirus Hepatitis C virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet3D

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HCV_core_protein: MSTNPKPQRKTKRNTNRRPQ DVKFPGGGQIVGGVYLLPRR GPRLGVRATRKTSERSQPRG RRQPIPKARRPEGRTWAQPG YPHHHHHH

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts48
1H chemical shifts48

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal half1

Entities:

Entity 1, N-terminal half 88 residues - Formula weight is not available

1   METSERTHRASNPROLYSPROGLNARGLYS
2   THRLYSARGASNTHRASNARGARGPROGLN
3   ASPVALLYSPHEPROGLYGLYGLYGLNILE
4   VALGLYGLYVALTYRLEULEUPROARGARG
5   GLYPROARGLEUGLYVALARGALATHRARG
6   LYSTHRSERGLUARGSERGLNPROARGGLY
7   ARGARGGLNPROILEPROLYSALAARGARG
8   PROGLUGLYARGTHRTRPALAGLNPROGLY
9   TYRPROHISHISHISHISHISHIS

Samples:

sample_1: HCV core protein, [U-100% 13C; U-100% 15N], 0.4 mM; Phosphate 25 mM; NaCl 50 mM; DSS 0.2 mM; NaN3 0.1%

sample_conditions_1: ionic strength: 75 mM; pH: 6.6; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

NMRView v5.2.2.01, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15768 4669
PDB
DBJ BAA00452 BAA00705 BAA00706 BAA00968 BAA01529
EMBL CAA03854 CAA43788 CAA43790 CAA43793 CAA46717
GB AAA16300 AAA16311 AAA20153 AAA20154 AAA21040
PIR JQ1926
REF NP_671491 YP_001469632
SP O39929 O92972 P26662 P26663 P26664
AlphaFold P26664 P26663 O39929 O92972 P26662

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks