BMRB Entry 15606

Title:
SOCS box elonginBC ternary complex
Deposition date:
2007-12-27
Original release date:
2008-07-29
Authors:
Babon, Jeffrey; Sabo, Jennifer; Soetopo, Alfreda; Yao, Shenggen; Bailey, Micheal; Zhang, Jian-guo; Nicola, Nicos; Norton, Raymond
Citation:

Citation: Babon, Jeffrey; Sabo, Jennifer; Soetopo, Alfreda; Yao, Shenggen; Bailey, Micheal; Zhang, Jian-Guo; Nicola, Nicos; Norton, Raymond. "The SOCS Box Domain of SOCS3: Structure and Interaction with the ElonginBC-Cullin5 Ubiquitin Ligase"  J. Mol. Biol. 381, 928-940 (2008).
PubMed: 18590740

Assembly members:

Assembly members:
socs_box, polymer, 40 residues, Formula weight is not available
elonginB, polymer, 118 residues, Formula weight is not available
elonginC, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T

Data sets:
Data typeCount
13C chemical shifts639
15N chemical shifts202
1H chemical shifts741

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1chain a1
2chain b2
3chain c3

Entities:

Entity 1, chain a 40 residues - Formula weight is not available

1   VALALATHRLEUGLNHISLEUCYSARGLYS
2   THRVALASNGLYHISLEUASPSERTYRGLU
3   LYSVALTHRGLNLEUPROGLYPROILEARG
4   GLUPHELEUASPGLNTYRASPALAPROLEU

Entity 2, chain b 118 residues - Formula weight is not available

1   METASPVALPHELEUMETILEARGARGHIS
2   LYSTHRTHRILEPHETHRASPALALYSGLU
3   SERSERTHRVALPHEGLULEULYSARGILE
4   VALGLUGLYILELEULYSARGPROPROASP
5   GLUGLNARGLEUTYRLYSASPASPGLNLEU
6   LEUASPASPGLYLYSTHRLEUGLYGLUCYS
7   GLYPHETHRSERGLNTHRALAARGPROGLN
8   ALAPROALATHRVALGLYLEUALAPHEARG
9   ALAASPASPTHRPHEGLUALALEUCYSILE
10   GLUPROPHESERSERPROPROGLULEUPRO
11   ASPVALMETLYSPROGLNASPSERGLYSER
12   SERALAASNGLUGLNALAVALGLN

Entity 3, chain c 96 residues - Formula weight is not available

1   METTYRVALLYSLEUILESERSERASPGLY
2   HISGLUPHEILEVALLYSARGGLUHISALA
3   LEUTHRSERGLYTHRILELYSALAMETLEU
4   SERGLYPROGLYGLNPHEALAGLUASNGLU
5   THRASNGLUVALASNPHEARGGLUILEPRO
6   SERHISVALLEUSERLYSVALCYSMETTYR
7   PHETHRTYRLYSVALARGTYRTHRASNSER
8   SERTHRGLUILEPROGLUPHEPROILEALA
9   PROGLUILEALALEUGLULEULEUMETALA
10   ALAASNPHELEUASPCYS

Samples:

sample_1: socs box, [U-100% 13C; U-100% 15N], 1 mM; elonginB 1 mM; elonginC 1 mM; potassium chloride 50 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6.7; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

TALOS, Cornilescu, Delaglio and Bax - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAA22430 BAA22537 BAC32977 BAD42435 BAE23929 BAB21946 BAB22417 BAB22684 BAB25690 BAB25727 BAB27825 BAB27939 BAB28546 BAB28641 BAC30437
EMBL CAB56083 CAG38736 CAG46495 CAL37774 CAL38024 CAG32006 CAJ81641 CBN81906 CDQ94726 CDQ97243
GB AAB62403 AAC26223 AAD42231 AAH52031 AAH60858 AAA75522 AAA80968 AAC08452 AAH13306 AAH51927 AAA41109 AAA67650 AAH09104 AAH13809 AAH28545
REF NP_001026801 NP_001116668 NP_001116851 NP_001181255 NP_003946 NP_001029972 NP_009039 NP_080581 NP_112391 NP_996896 NP_001002440 NP_001004673 NP_001007889 NP_001016420 NP_001039958
SP O14543 O35718 O88583 Q68AM8 Q9BEG9 P62869 P62870 Q15370 P83940 P83941 Q15369 Q2KII4
TPG DAA18184 DAA15620 DAA22672
BMRB 19333 19333
PRF 2119399A 2119399B
AlphaFold O14543 O35718 O88583 Q68AM8 Q9BEG9 Q15370 P62869 P62870 Q2KII4 Q15369 P83941 P83940

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks