BMRB Entry 11440

Title:
Solution structure of Magnesium-bound form of calmodulin C-domain E104D/E140D mutant
Deposition date:
2011-04-27
Original release date:
2011-08-19
Authors:
Hirota, Hiroshi
Citation:

Citation: Ohashi, Wakana; Hirota, Hiroshi; Yamazaki, Toshio. "Solution structure and fluctuation of the Mg(2+) -bound form of calmodulin C-terminal domain."  Protein Sci. 20, 690-701 (2011).
PubMed: 21312310

Assembly members:

Assembly members:
entity, polymer, 72 residues, 8259.024 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts66
1H chemical shifts468

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mg(2+) -bound form of calmodulin C-terminal domain1

Entities:

Entity 1, Mg(2+) -bound form of calmodulin C-terminal domain 72 residues - 8259.024 Da.

1   METASPTHRASPSERGLUGLUGLUILEARG
2   GLUALAPHEARGVALPHEASPLYSASPGLY
3   ASNGLYTYRILESERALAALAASPLEUARG
4   HISVALMETTHRASNLEUGLYGLULYSLEU
5   THRASPGLUGLUVALASPGLUMETILEARG
6   GLUALAASPILEASPGLYASPGLYGLNVAL
7   ASNTYRGLUASPPHEVALGLNMETMETTHR
8   ALALYS

Samples:

sample_1: MES, [U-2H], 10 mM; potassium chloride 100 mM; magnesium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.5 M; pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CYANA v2.2, Guntert, Mumenthaler and Wuthrich - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

xwinnmr, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 17881 17981 17982 17983 18084 18323 25344
PDB
DBJ BAF45809 BAI66111 BAI66112 BAI66113 BAI66114
GB AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961
REF XP_007442524 XP_010190430 XP_011906627 XP_014456117

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks