BMRB Entry 10016

Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for the Nucleotide-Free Form of Human ABCB6 C-Terminal Domain
Deposition date:
2005-12-08
Original release date:
2006-06-27
Authors:
Kurashima-Ito, Kaori; Ikeya, Teppei; Senbongi, Hiroshi; Tochio, Hidehito; Mikawa, Tsutomu; Shibata, Takehiko; Ito, Yutaka
Citation:

Citation: Kurashima-Ito, Kaori; Ikeya, Teppei; Senbongi, Hiroshi; Tochio, Hidehito; Mikawa, Tsutomu; Shibata, Takehiko; Ito, Yutaka. "Heteronuclear multidimensional NMR and homology modelling studies of the C-terminal nucleotide-binding domain of the human mitochondrial ABC transporter ABCB6"  J. Biomol. NMR 35, 53-71 (2006).
PubMed: 16791740

Assembly members:

Assembly members:
the C-terminal nucleotide-binding domain of the human mitochondrial ABC transporter ABCB6, polymer, 285 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts1016
15N chemical shifts368
1H chemical shifts368

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ABCB6-C1

Entities:

Entity 1, ABCB6-C 285 residues - Formula weight is not available

residues from Phe558 to the C-terminal Arg842

1   PHEILEASPMETGLUASNMETPHEASPLEU
2   LEULYSGLUGLUTHRGLUVALLYSASPLEU
3   PROGLYALAGLYPROLEUARGPHEGLNLYS
4   GLYARGILEGLUPHEGLUASNVALHISPHE
5   SERTYRALAASPGLYARGGLUTHRLEUGLN
6   ASPVALSERPHETHRVALMETPROGLYGLN
7   THRLEUALALEUVALGLYPROSERGLYALA
8   GLYLYSSERTHRILELEUARGLEULEUPHE
9   ARGPHETYRASPILESERSERGLYCYSILE
10   ARGILEASPGLYGLNASPILESERGLNVAL
11   THRGLNALASERLEUARGSERHISILEGLY
12   VALVALPROGLNASPTHRVALLEUPHEASN
13   ASPTHRILEALAASPASNILEARGTYRGLY
14   ARGVALTHRALAGLYASNASPGLUVALGLU
15   ALAALAALAGLNALAALAGLYILEHISASP
16   ALAILEMETALAPHEPROGLUGLYTYRARG
17   THRGLNVALGLYGLUARGGLYLEULYSLEU
18   SERGLYGLYGLULYSGLNARGVALALAILE
19   ALAARGTHRILELEULYSALAPROGLYILE
20   ILELEULEUASPGLUALATHRSERALALEU
21   ASPTHRSERASNGLUARGALAILEGLNALA
22   SERLEUALALYSVALCYSALAASNARGTHR
23   THRILEVALVALALAHISARGLEUSERTHR
24   VALVALASNALAASPGLNILELEUVALILE
25   LYSASPGLYCYSILEVALGLUARGGLYARG
26   HISGLUALALEULEUSERARGGLYGLYVAL
27   TYRALAASPMETTRPGLNLEUGLNGLNGLY
28   GLNGLUGLUTHRSERGLUASPTHRLYSPRO
29   GLNTHRMETGLUARG

Samples:

sample_1: human ABCB6-C in the nucleotide-free form, [U-2H; U-13C; U-15N], 0.2 mM

condition_1: pH: 8.5; temperature: 298 K

condition_2: pH: 7.5; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
1H-15N TROSY-HSQCsample_1not availablenot available
TROSY-HNCAsample_1not availablenot available
TROSY-HN(CO)CAsample_1not availablenot available
TROSY-HN(CA)CBsample_1not availablenot available
TROSY-HN(COCA)CBsample_1not availablenot available
TROSY-HN(CA)COsample_1not availablenot available
TRSOY-HNCOsample_1not availablenot available

Software:

XWIN-NMR v3.5, Bruker - collection

AZARA v2.7, Wayne Boucher & Dept. Biochemistry, Univ. Cambridge - processing

ANSIG-for-OpenGL v1.0.6, Per Kraulis - assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 10017
PDB
DBJ BAA96733 BAB71347 BAD18782 BAK62401 BAK62910
EMBL CAB95766
GB AAC28653 AAF75107 AAG33617 AAH00559 AAH43423
REF NP_005680 XP_001161097 XP_002799099 XP_002799100 XP_002812920
SP Q9NP58
AlphaFold Q9NP58

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks