BMRB Entry 5388
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5388
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Citation: Liu, Qin; Li, Shawn. "Letter to the Editor: 1H,13C and 15N resonance assignments of Gads C-terminal
SH3 domain in complex with an RXXK motif-containing peptide derived from
SLP-76" J. Biomol. NMR 24, 161-162 (2002).
Assembly members:
Gads C-terminal SH3, polymer, 75 residues, 8409 Da.
peptide of SLP76, polymer, 11 residues, 1142 Da.
Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Gads C-terminal SH3: GSRRASVGSVQLQAAGRVRW
ARALYDFEALEEDELGFRSG
EVVEVLDSSNPSWWTGRLHN
KLGLFPANYVAPMMR
peptide of SLP76: APSIDRSTKPA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 578 |
| 13C chemical shifts | 355 |
| 15N chemical shifts | 87 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | c-Gads SH3 | 1 |
| 2 | peptide of SLP76 | 2 |
Entities:
Entity 1, c-Gads SH3 75 residues - 8409 Da.
| 1 | GLY | SER | ARG | ARG | ALA | SER | VAL | GLY | SER | VAL | ||||
| 2 | GLN | LEU | GLN | ALA | ALA | GLY | ARG | VAL | ARG | TRP | ||||
| 3 | ALA | ARG | ALA | LEU | TYR | ASP | PHE | GLU | ALA | LEU | ||||
| 4 | GLU | GLU | ASP | GLU | LEU | GLY | PHE | ARG | SER | GLY | ||||
| 5 | GLU | VAL | VAL | GLU | VAL | LEU | ASP | SER | SER | ASN | ||||
| 6 | PRO | SER | TRP | TRP | THR | GLY | ARG | LEU | HIS | ASN | ||||
| 7 | LYS | LEU | GLY | LEU | PHE | PRO | ALA | ASN | TYR | VAL | ||||
| 8 | ALA | PRO | MET | MET | ARG |
Entity 2, peptide of SLP76 11 residues - 1142 Da.
| 1 | ALA | PRO | SER | ILE | ASP | ARG | SER | THR | LYS | PRO | ||||
| 2 | ALA |
Samples:
sample_1: Gads C-terminal SH3, [U-13C; U-15N], 1.0 mM
sample_2: peptide of SLP76, [U-13C; U-15N], 1.0 mM
Ex-cond_1: pH: 6.0; temperature: 285 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H,15N HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCACONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| 13C,15N NOESY-HSQC | not available | not available | not available |
| half-filtered NOESY-HSQC | not available | not available | not available |
| HCC(CO)NH | not available | not available | not available |
| CC(CO)NH | not available | not available | not available |
| (HB)CB(CGCD)HD | not available | not available | not available |
| (HB)CB(CGCDCE)HE | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks