BMRB Entry 5102

Title:
A helical region in the C-terminus of small-conductance Ca2+-activated K+ channels controls assembly with apo-calmodulin
Deposition date:
2001-08-08
Original release date:
2002-02-04
Authors:
Wissmann, Ralph; Bildl, Wolfgang; Neumann, Heinz; Rivard, Andre; Kloecker, Nikolaj; Weitz, Dietmar; Schulte, Uwe; Adelman, John; Bentrop, Detlef; Fakler, Bernd
Citation:

Citation: Wissmann, Ralph; Bildl, Wolfgang; Neumann, Heinz; Rivard, Andre; Kloecker, Nikolaj; Weitz, Dietmar; Schulte, Uwe; Adelman, John; Bentrop, Detlef; Fakler, Bernd. "A helical region in the C-terminus of small-conductance Ca2+-activated K+ channels controls assembly with apo-calmodulin"  J. Biol. Chem. 277, 4558-4564 (2002).

Assembly members:

Assembly members:
SK2 calmodulin binding domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET23B

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts342
15N chemical shifts343
1H chemical shifts871
coupling constants37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CaMBD1

Entities:

Entity 1, CaMBD 102 residues - Formula weight is not available

1   METGLYARGLYSLEUGLULEUTHRLYSALA
2   GLULYSHISVALHISASNPHEMETMETASP
3   THRGLNLEUTHRLYSARGVALLYSASNALA
4   ALAALAASNVALLEUARGGLUTHRTRPLEU
5   ILETYRLYSASNTHRLYSLEUVALLYSLYS
6   ILEASPHISALALYSVALARGLYSHISGLN
7   ARGLYSPHELEUGLNALAILEHISGLNLEU
8   ARGSERVALLYSMETGLUGLNARGLYSLEU
9   ASNASPGLNALAASNTHRLEUVALASPLEU
10   ALALYSTHRGLNLEUGLUHISHISHISHIS
11   HISHIS

Samples:

sample_1: SK2 calmodulin binding domain, [U-98% 15N], 0.5 – 1.2 mM; NaCl 250 mM

sample_2: SK2 calmodulin binding domain, [U-98% 13C; U-98% 15N], 0.5 – 1.0 mM; NaCl 250 mM

Ex-cond_1: ionic strength: 0.25 M; pH: 3.5 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAC28176 BAC34231 BAD08234 BAE28732 BAF82637
EMBL CAH90265
GB AAB09563 AAF21783 AAG16728 AAH15371 AAI17455
REF NP_001125117 NP_001265133 NP_062187 NP_067627 NP_536713
SP P58390 P70604 Q9H2S1
TPG DAA25921
AlphaFold P58390 P70604 Q9H2S1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks