BMRB Entry 4965

Title:
1H, 15N, and 13C Assignments and Secondary Structure Identification for Full-length Ribosomal Protein L11 from Thermus thermophilus
Deposition date:
2001-03-05
Original release date:
2006-10-06
Authors:
Markus, Michelle; Triantafillidou, Dimitra; Choli-Papadopoulou, Theodora; Torchia, Dennis
Citation:

Citation: Markus, Michelle; Triantafillidou, Dimitra; Choli-Papadopoulou, Theodora; Torchia, Dennis. "Letter to the Editor: 1H, 15N, and 13C Assignments and Secondary Structure Identification for Full-length Ribosomal Protein L11 from Thermus thermophilus"  J. Biomol. NMR 20, 293-294 (2001).
PubMed: 11519754

Assembly members:

Assembly members:
ribosomal protein L11, polymer, 147 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
1H chemical shifts941
13C chemical shifts635
15N chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tth L111

Entities:

Entity 1, Tth L11 147 residues - Formula weight is not available

1   METLYSLYSVALVALALAVALVALLYSLEU
2   GLNLEUPROALAGLYLYSALATHRPROALA
3   PROPROVALGLYPROALALEUGLYGLNHIS
4   GLYALAASNILEMETGLUPHEVALLYSALA
5   PHEASNALAALATHRALAASNMETGLYASP
6   ALAILEVALPROVALGLUILETHRILETYR
7   ALAASPARGSERPHETHRPHEVALTHRLYS
8   THRPROPROALASERTYRLEUILEARGLYS
9   ALAALAGLYLEUGLULYSGLYALAHISLYS
10   PROGLYARGGLULYSVALGLYARGILETHR
11   TRPGLUGLNVALLEUGLUILEALALYSGLN
12   LYSMETPROASPLEUASNTHRTHRASPLEU
13   GLUALAALAALAARGMETILEALAGLYSER
14   ALAARGSERMETGLYVALGLUVALVALGLY
15   ALAPROGLUVALLYSASPALA

Samples:

15N_sample: ribosomal protein L11, [U-99% 15N], 1.06 mM; KH2PO4 10 mM; KCl 70 mM; sodium azide 0.1 mM

13C_15N_sample: ribosomal protein L11, [U-99% 15N; U-95% 13C], 1.12 mM; KH2PO4 10 mM; KCl 70 mM; sodium azide 0.1 mM

13C_15N_in_D2O: ribosomal protein L11, [U-99% 15N; U-95% 13C], 0.886 mM; D2O 100%; KH2PO4 10 mM; KCl 70 mM; sodium azide 0.1 mM

NMR_buffer: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
HCACOnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HAHBnot availablenot availablenot available
LRCCnot availablenot availablenot available
2D 1H-1H TOCSYnot availablenot availablenot available
13C-separated NOESYnot availablenot availablenot available
15N-separated NOESYnot availablenot availablenot available

Software:

nmrDraw - data processing

nmrPipe - data processing

PIPP - interactive peak-picking

NMR spectrometers:

  • DMX Bruker 500 MHz
  • DMX Bruker 750 MHz

Related Database Links:

BMRB 7314 7315
PDB
DBJ BAD70070
EMBL CAA57138
GB AAS82080 ADW20820 AEG32574 AEV17153 AFH38065
PIR S66576
PRF 2004301A
REF WP_008630514 WP_011174097 WP_014516502 WP_014628913 WP_015716108
SP P36238 P62442 Q5SLP6
AlphaFold P62442 P36238 Q5SLP6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks