BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4925

Title: Structural Basis for the Functional switch of the E. Coli Ada Protein   PubMed: 11284682

Authors: Lin, Yingxi; Dotsch, Volker; Wintner, T.; Peariso, Katrina; Myers, Lawrence; Penner-Hahn, James; Verdine, Gregory; Wagner, Gerhard

Citation: Lin, Yingxi; Dotsch, Volker; Wintner, T.; Peariso, Katrina; Myers, Lawrence; Penner-Hahn, James; Verdine, Gregory; Wagner, Gerhard. "Structural Basis for the Functional switch of the E. Coli Ada Protein"  Biochemistry 40, 4261-4271 (2001).

Assembly members:
N-Ada10, polymer, 92 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
N-Ada10: MKKATCLTDDQRWQSVLARD PNADGEFVFAVRTTGIFCRP SCRARHALRENVSFYANASE ALAAGFRPCKRCQPDKANPR QHRLDKITHACR

Data sets:
Data typeCount
1H chemical shifts556
13C chemical shifts85
15N chemical shifts90

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Ada10 subunit 11
2Zn2+2

Entities:

Entity 1, N-Ada10 subunit 1 92 residues - Formula weight is not available

1   METLYSLYSALATHRCYSLEUTHRASPASP
2   GLNARGTRPGLNSERVALLEUALAARGASP
3   PROASNALAASPGLYGLUPHEVALPHEALA
4   VALARGTHRTHRGLYILEPHECYSARGPRO
5   SERCYSARGALAARGHISALALEUARGGLU
6   ASNVALSERPHETYRALAASNALASERGLU
7   ALALEUALAALAGLYPHEARGPROCYSLYS
8   ARGCYSGLNPROASPLYSALAASNPROARG
9   GLNHISARGLEUASPLYSILETHRHISALA
10   CYSARG

Entity 2, Zn2+ - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: N-Ada10, [U-15N; U-13C], 2 – 3 mM

Ex-cond1: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond1
1H-15N TOCSYsample_1not availableEx-cond1
2D NOESYsample_1not availableEx-cond1
HCCH-TOCSYsample_1not availableEx-cond1

Software:

No software information available

NMR spectrometers:

  • Varian Unity-plus 750 MHz
  • Varian Unity-plus 450 MHz
  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

REF YP_670153 YP_408500 YP_311153 NP_837823 NP_708108
GenBank ABB66672 AAZ88918 AAP17632 AAN43815 AAA23413
PDB
BMRB 6605