BMRB Entry 4393

Title:
1H, 15N, and 13C NMR Backbone Assignments of the N Terminal Region of Human Erythrocyte Alpha Spectrin Including One Repeating Unit.
Deposition date:
1999-09-08
Original release date:
2003-06-05
Authors:
Park, Sunghyouk; Liao, Xiubei; Johnson, Michael; Fung, Leslie
Citation:

Citation: Park, Sunghyouk; Liao, Xiubei; Johnson, Michael; Fung, Leslie. "1H, 15N, and 13C NMR Backbone Assignments of the N-terminal Region of Human Erythrocyte alpha Spectrin Including one Structural Domain"  J. Biomol. NMR 15, 345-346 (1999).
PubMed: 10685345

Assembly members:

Assembly members:
spectrin, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts301
15N chemical shifts149
1H chemical shifts298
coupling constants96

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1spectrin1

Entities:

Entity 1, spectrin 156 residues - Formula weight is not available

1   METGLUGLNPHEPROLYSGLUTHRVALVAL
2   GLUSERSERGLYPROLYSVALLEUGLUTHR
3   ALAGLUGLUILEGLNGLUARGARGGLNGLU
4   VALLEUTHRARGTYRGLNSERPHELYSGLU
5   ARGVALALAGLUARGGLYGLNLYSLEUGLU
6   ASPSERTYRHISLEUGLNVALPHELYSARG
7   ASPALAASPASPLEUGLYLYSTRPILEMET
8   GLULYSVALASNILELEUTHRASPLYSSER
9   TYRGLUASPPROTHRASNILEGLNGLYLYS
10   TYRGLNLYSHISGLNSERLEUGLUALAGLU
11   VALGLNTHRLYSSERARGLEUMETSERGLU
12   LEUGLULYSTHRARGGLUGLUARGPHETHR
13   METGLYHISSERALAHISGLUGLUTHRLYS
14   ALAHISILEGLUGLULEUARGHISLEUTRP
15   ASPLEULEULEUGLULEUTHRLEUGLULYS
16   GLYASPGLNLEULEUARG

Samples:

sample_one: spectrin, [U-2H; U-15N; U-13C], 0.9 – 1.1 mM; Phosphate Buffer 5 mM; Sodium Chloride 150 mM

sample_conditions: ionic strength: 0.18 M; pH: 6.5 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600.13 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks