BMRB Entry 4267

Title:
Chemical shift assignments, 3JHNHA coupling constants and secondary structure of HNGAL (Human Neutrophil Gelatinase-Associated Lipocalin) in its apo form.
Deposition date:
1998-11-11
Original release date:
2011-03-03
Authors:
Coles, Murray; Diercks, Tammo; Muehlenweg, Bernd; Bartsch, Stefan; Zoelzer, Volker; Tschesche, Harald; Kessler, Horst
Citation:

Citation: Coles, Murray; Diercks, Tammo; Muehlenweg, Bernd; Bartsch, Stefan; Zoelzer, Volker; Tschesche, Harald; Kessler, Horst. "The Solution Strucuture and Dynamics of Human Neutrophil Gelatinase-associated Lipocalin."  J. Mol. Biol. 289, 139-157 (1999).

Assembly members:

Assembly members:
HNGAL Human Neutrophil Gelatinase-Associated Lipocalin, polymer, 179 residues, 20660 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Bl21[DE3] Escherichia   Vector: pET11a

Data typeCount
13C chemical shifts737
15N chemical shifts188
1H chemical shifts1266
coupling constants125
order parameters152
T1 relaxation values443
T2 relaxation values445

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HNGAL1

Entities:

Entity 1, HNGAL 179 residues - 20660 Da.

1   METGLNASPSERTHRSERASPLEUILEPRO
2   ALAPROPROLEUSERLYSVALPROLEUGLN
3   GLNASNPHEGLNASPASNGLNPHEGLNGLY
4   LYSTRPTYRVALVALGLYLEUALAGLYASN
5   ALAILELEUARGGLUASPLYSASPPROGLN
6   LYSMETTYRALATHRILETYRGLULEULYS
7   GLUASPLYSSERTYRASNVALTHRSERVAL
8   LEUPHEARGLYSLYSLYSCYSASPTYRTRP
9   ILEARGTHRPHEVALPROGLYCYSGLNPRO
10   GLYGLUPHETHRLEUGLYASNILELYSSER
11   TYRPROGLYLEUTHRSERTYRLEUVALARG
12   VALVALSERTHRASNTYRASNGLNHISALA
13   METVALPHEPHELYSLYSVALSERGLNASN
14   ARGGLUTYRPHELYSILETHRLEUTYRGLY
15   ARGTHRLYSGLULEUTHRSERGLULEULYS
16   GLUASNPHEILEARGPHESERLYSSERLEU
17   GLYLEUPROGLUASNHISILEVALPHEPRO
18   VALPROILEASPGLNCYSILEASPGLY

Samples:

HNGAL_sample_N15: HNGAL Human Neutrophil Gelatinase-Associated Lipocalin, [U-15N], 1.43 mM; sodium acetate 50 mM; acetic acid 50 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O 10.0%; H2O 90.0%

HNGAL_sample_dl: HNGAL Human Neutrophil Gelatinase-Associated Lipocalin, [U-15N; U-13C], 1.15 mM; sodium acetate 50 mM; acetic acid 50 mM; sodium chloride 50 mM; sodium azide 0.02%; D2O 10.0%; H2O 90.0%

sample_conditions_all: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_all

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAG63166 BAH14588 BAJ21166
EMBL CAA58127 CAA67574 CAG46889
GB AAB26529 AAD14168 AAH33089 AAV38204 AAX36313
REF NP_005555 XP_003822366 XP_004048725 XP_520287
SP P80188
AlphaFold P80188

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks