BMRB Entry 4082

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4082

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Title:
Characterization of the Three-Dimensional Solution Structure of Human Profilin: 1H, 13C, and 15N NMR Assignments amd Global Folding Pattern
Deposition date:
1997-12-22
Original release date:
2001-02-17
Authors:
Metzler, William; Constantine, Keith; Friedrichs, Mark; Bell, Aneka; Ernst, Eileen; Lavoie, Thomas; Mueller, Luciano
Citation:

Citation: Metzler, William; Constantine, Keith; Friedrichs, Mark; Bell, Aneka; Ernst, Eileen; Lavoie, Thomas; Mueller, Luciano. "Characterization of the Three-Dimensional Solution Structure of Human Profilin: 1H, 13C, and 15N Assignments and Global Folding Pattern"  Biochemistry 32, 13818-13829 (1993).

Assembly members:

Assembly members:
profilin, polymer, 139 residues, 15000 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGTL-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
profilin: AGWNAYIDNLMADGTCQDAA IVGYKDSPSVWAAVPGKTFV NITPAEVGVLVGKDRSSFYV NGLTLGGQKCSVIRDSLLQD GEFSMDLRTKSTGGAPTFNV TVTKTDKTLVLLMGKEGVHG GLINKKCYEMASHLRRSQY

Data sets:
Data typeCount
13C chemical shifts440
15N chemical shifts144
1H chemical shifts937

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1profilin1

Entities:

Entity 1, profilin 139 residues - 15000 Da.

1   ALAGLYTRPASNALATYRILEASPASNLEU
2   METALAASPGLYTHRCYSGLNASPALAALA
3   ILEVALGLYTYRLYSASPSERPROSERVAL
4   TRPALAALAVALPROGLYLYSTHRPHEVAL
5   ASNILETHRPROALAGLUVALGLYVALLEU
6   VALGLYLYSASPARGSERSERPHETYRVAL
7   ASNGLYLEUTHRLEUGLYGLYGLNLYSCYS
8   SERVALILEARGASPSERLEULEUGLNASP
9   GLYGLUPHESERMETASPLEUARGTHRLYS
10   SERTHRGLYGLYALAPROTHRPHEASNVAL
11   THRVALTHRLYSTHRASPLYSTHRLEUVAL
12   LEULEUMETGLYLYSGLUGLYVALHISGLY
13   GLYLEUILEASNLYSLYSCYSTYRGLUMET
14   ALASERHISLEUARGARGSERGLNTYR

Samples:

sample_one: profilin, [U-15N], 2.0 mM; phosphate 10 mM; KCl 25 mM; B-mercaptoethanol 20 mM; EDTA 1.0 mM; H2O 90%; D2O 10%

sample_two: profilin, [U-15N], 2.0 mM; phosphate 10 mM; KCl 25 mM; B-mercaptoethanol 20 mM; EDTA 1.0 mM; D2O 100%

sample_three: profilin, [U-13C], 2.0 mM; phosphate 10 mM; KCl 25 mM; B-mercaptoethanol 20 mM; EDTA 1.0 mM; H2O 90%; D2O 10%

sample_four: profilin, [U-13C], 2.0 mM; phosphate 10 mM; KCl 25 mM; B-mercaptoethanol 20 mM; EDTA 1.0 mM; D2O 100%

sample_five: profilin, [U-15N], [U-13C], 2.0 mM; phosphate 10 mM; KCl 25 mM; B-mercaptoethanol 20 mM; EDTA 1.0 mM; H2O 90%; D2O 10%

sample_six: profilin, [U-15N], [U-13C], 2.0 mM; phosphate 10 mM; KCl 25 mM; B-mercaptoethanol 20 mM; EDTA 1.0 mM; D2O 100%

sample_conditions: pH: 6.4; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

PDB
DBJ BAE87684 BAG35102 BAG73580
EMBL CAG28598
GB AAA36486 AAH02475 AAH06768 AAH13439 AAH15164
REF NP_001270319 NP_005013 XP_001099416 XP_002800300 XP_003733022
SP P07737
AlphaFold P07737

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks